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The catalytic efficiency of two different enzymes can be compared by the

1. formation of the product
2. pH optimum value
3. Km value
4. molecular size of the enzyme

Correct answer: Km value

Solution

Km is the substrate concentration at which an enzyme works at half its maximum rate, so it reflects substrate affinity and is commonly used to compare catalytic efficiency. A lower Km generally means higher affinity and often greater efficiency under the same conditions.

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