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The \( 3^{\prime}-5^{\prime} \) phosphodiester linkages inside a polynucleotide chain serve to join

1. One nitrogenous base with pentose sugar
2. One DNA strand with the other DNA strand
3. one nucleoside with another nucleoside
4. One nucleotide with another nucleotide

Correct answer: One nucleotide with another nucleotide

Solution

A 3′-5′ phosphodiester bond forms between the phosphate of one nucleotide and the 3′ hydroxyl of the next sugar, creating the backbone of DNA or RNA. Therefore, it joins one nucleotide to another nucleotide.

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