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All the reactions in glycolysis are reversible except

1. 3 -Phosphoglycerate to 1,3 -Biphosphoglycerate.
2. Glucose 6-phosphate to fructose - 6-phosphate.
3. 1,3 -Biphosphoglycerate to glyceraldehyde 3 -P.
4. Fructose - 6 -phosphate to fructose- 1,6 -diphosphate.

Correct answer: Fructose - 6 -phosphate to fructose- 1,6 -diphosphate.

Solution

Most glycolytic reactions are near-equilibrium and can run in either direction. The phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate is strongly exergonic and is essentially irreversible under cellular conditions.

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