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Which one of the following statements is correct, with reference to enzymes?

1. Holoenzyme = Apoenzyme + Coenzyme
2. Coenzyme = Apoenzyme + Holoenzyme
3. Holoenzyme = Coenzyme + Co-factor
4. Apoenzyme = Holoenzyme + Coenzyme

Correct answer: Holoenzyme = Apoenzyme + Coenzyme

Solution

A holoenzyme is the fully active enzyme complex formed when the protein part (apoenzyme) combines with its required coenzyme or cofactor. The apoenzyme alone is inactive, so the correct relationship is apoenzyme + coenzyme = holoenzyme.

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