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Prosthetic groups differ from co-enzymes in that-

1. They can serve as co-factors in a number of enzyme-catalysed reactions.
2. They require metal ions for their activity.
3. They (prosthetic groups) are tightly bound to apoenzymes.
4. Their association with apoenzymes is transient.

Correct answer: They (prosthetic groups) are tightly bound to apoenzymes.

Solution

Prosthetic groups are non-protein components that remain tightly bound to the apoenzyme, often permanently. Coenzymes, in contrast, are usually loosely or transiently associated and can move between enzymes.

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