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Proline (symbol Pro or P) is a proteinogenic amino acid that is used in the biosynthesis of proteins.

1. Proline is the only proteinogenic amino acid with a secondary amine.
2. The alpha-amino group is attached directly to the main chain.
3. Proline is unique in that it is the only amino acid where the side chain is connected to the protein backbone twice.
4. Proline can be considered to be an opposite of Glycine.

Correct answer: Proline is unique in that it is the only amino acid where the side chain is connected to the protein backbone twice.

Solution

Proline is special because its side chain cyclizes back onto the backbone nitrogen, making a ring and connecting to the backbone twice. That structural constraint is what makes the correct statement true.

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