PCR is based on three simple steps required for any DNA synthesis reaction: (i) denaturation of the template into single strands; (ii) annealing of primers to each original strand for new strand synthesis; and (iii) extension of the new DNA strands from the primers.

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PCR involves three steps.. PCR requires three core stages in each cycle: the DNA is first denatured into single strands, primers anneal to the templates, and DNA polymerase extends from the primers. Since the process is explicitly described as having these three steps, the correct choice is three steps.

PCR is based on three simple steps required for any DNA synthesis reaction: (i) denaturation of the template into single strands; (ii) annealing of primers to each original strand for new strand synthesis; and (iii) extension of the new DNA strands from the primers.

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