Exams › NEET › Biology

Which one is a true statement regarding DNA polymerase used in PCR

1. It is used to ligate introduced DNA in recipient cell
2. It serves as a selectable marker
3. It is isolated from a virus
4. It remains active at high temperature

Correct answer: It remains active at high temperature

Solution

PCR repeatedly heats the reaction to separate DNA strands, so the polymerase must not denature at those temperatures. Thermostable DNA polymerases, such as Taq polymerase, remain active after high-heat steps and can synthesize new DNA strands.

Related NEET Biology questions

• The sequence that controls the copy number of the linked DNA in the vector, is termed:
• For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:
• Biolistics (gene-gun) is suitable for:
• PCR and Restriction Fragment Length Polymorphism are the methods for:
• The separated DNA fragments can be visualised only after staining the DNA with Ethidium bromide followed by exposure to UV radiation.
• Given below are four statements pertaining to separation of DNA fragments using Gel electrophoresis. Identify the incorrect statements. (i) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal. (ii) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (iii) Smaller the size of DNA fragment, larger is the distance it travels through it. (iv) Pure DNA can be visualised directly by exposing to UV radiation. Choose correct answer from the options given below:

⚔️ Practice NEET Biology free + battle 1v1 →