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Given below are four statements pertaining to separation of DNA fragments using Gel electrophoresis. Identify the incorrect statements. (i) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal. (ii) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (iii) Smaller the size of DNA fragment, larger is the distance it travels through it. (iv) Pure DNA can be visualised directly by exposing to UV radiation. Choose correct answer from the options given below:

1. (a) (i), (ii) and (iv)
2. (b) (i), (iii) and (iv)
3. (c) (i), (ii) and (iii)
4. (d) (ii), (iii) and (iv)

Correct answer: (a) (i), (ii) and (iv)

Solution

DNA is negatively charged, so it migrates toward the anode, but it is loaded near the cathode, making (i) incorrect. DNA moves through the gel matrix and its movement depends on gel concentration, and pure DNA is not directly visible under UV without staining, so (ii) and (iv) are also incorrect.

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