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A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Each restriction endonuclease functions by inspecting the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar phosphate backbone. These enzymes predictably cut both strands because the sequences they recognize are palindromic. That is the recognition sequences of identical bases on both DNA strands.

1. Restriction enzymes cut DNA at random sites.
2. Restriction enzymes cut DNA at specific recognition sites.
3. Restriction enzymes cut RNA at specific recognition sites.
4. Restriction enzymes cut RNA at random sites.

Correct answer: Restriction enzymes cut DNA at specific recognition sites.

Solution

Restriction enzymes are sequence-specific endonucleases, meaning they identify a particular DNA recognition site and cut there. The correct choice matches both the DNA target and the specificity of the cut.

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