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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:

1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation

Correct answer: Electrophoresis

Solution

Electrophoresis separates DNA fragments based on size because DNA is negatively charged and moves through a gel toward the positive electrode. Smaller fragments travel farther, making it ideal for analyzing restriction enzyme products.

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