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The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)?

1. ori - original restriction enzyme
2. rop - reduced osmotic pressure
3. Hind III, EcoRI - selectable markers
4. ampR, tetR - antibiotic resistance genes

Correct answer: ampR, tetR - antibiotic resistance genes

Solution

In pBR322, ampR and tetR are the selectable marker genes because they confer resistance to ampicillin and tetracycline. The other options confuse the origin of replication, a regulatory gene, or restriction sites with marker genes.

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