Exams › NEET › Biology

The linking of antibiotic resistance gene with the plasmid vector became possible with:

1. DNA ligase
2. Endonucleases
3. DNA polymerase
4. Exonucleases

Correct answer: DNA ligase

Solution

DNA ligase joins DNA fragments by sealing breaks in the sugar-phosphate backbone, allowing the resistance gene to be inserted into the plasmid vector. Restriction enzymes cut DNA, but ligase is needed to link the pieces permanently.

Related NEET Biology questions

• The sequence that controls the copy number of the linked DNA in the vector, is termed:
• For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:
• Biolistics (gene-gun) is suitable for:
• PCR and Restriction Fragment Length Polymorphism are the methods for:
• Which one is a true statement regarding DNA polymerase used in PCR
• The separated DNA fragments can be visualised only after staining the DNA with Ethidium bromide followed by exposure to UV radiation.

⚔️ Practice NEET Biology free + battle 1v1 →