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Restriction endonucleases are enzymes which:

1. make cuts at specific positions within the DNA molecule
2. recognize a specific nucleotide sequence for binding of DNA ligase
3. restrict the action of the enzyme DNA polymerase
4. remove nucleotides from the ends of the DNA molecule

Correct answer: make cuts at specific positions within the DNA molecule

Solution

Restriction endonucleases are enzymes that cut DNA at specific recognition sequences, producing fragments of predictable sizes. They do not bind ligase, block polymerase, or trim nucleotides from DNA ends as their main function.

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