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Polyethylene glycol method is used for:

1. biodiesel production
2. seedless fruit production
3. energy production from sewage
4. gene transfer without a vector

Correct answer: gene transfer without a vector

Solution

Polyethylene glycol (PEG) is commonly used to facilitate direct uptake of DNA into cells, especially in protoplasts, by promoting membrane fusion and transient permeability. This makes it useful for gene transfer without using a vector.

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