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The correct order of steps in Polymerase Chain Reaction (PCR) is

1. Extension, Denaturation, Annealing
2. Annealing, Extension, Denaturation
3. Denaturation, Annealing, Extension
4. Denaturation, Extension, Annealing

Correct answer: Denaturation, Annealing, Extension

Solution

PCR begins by heating to separate the double-stranded DNA, which is denaturation. Next, the temperature is lowered so primers can bind to the template during annealing. Finally, DNA polymerase extends the primers to synthesize new strands.

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