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Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

1. 5'..........CGTTGC..........3' 3'..........GCAACG..........5'
2. 5'..........AGGTGA..........3' 3'..........TCCACT..........5'
3. 5'..........GATATC..........3' 3'..........CTATAG..........5'
4. 5'..........GAATTC..........3' 3'..........CTTAAG..........5'

Correct answer: 5'..........GAATTC..........3' 3'..........CTTAAG..........5'

Solution

GAATTC is the recognition site for EcoRI, a restriction endonuclease that cuts within the palindrome near the center. The other sequences are either not standard sites or do not match a well-known enzyme cut pattern.

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