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An analysis of chromosomal DNA using the Southern hybridization technique does not use:

1. Electrophoresis
2. Blotting
3. Autoradiography
4. PCR

Correct answer: PCR

Solution

Southern hybridization involves restriction digestion, electrophoresis, blotting to a membrane, and detection with a labeled probe, often visualized by autoradiography. PCR is a separate DNA amplification method and is not required for Southern blot analysis.

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