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The cutting of DNA at specific locations became possible with the discovery of

1. Probes
2. Selectable markers
3. Ligases
4. Restriction enzymes

Correct answer: Restriction enzymes

Solution

Restriction enzymes are endonucleases that recognize specific DNA sequences and cleave the DNA at or near those sites. This made precise cutting of DNA possible, unlike probes, selectable markers, or ligases.

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