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The DNA fragments separated on an agarose gel can be visualised after staining with:

1. Acetocarmine
2. Aniline blue
3. Ethidium bromide
4. Bromophenol blue

Correct answer: Ethidium bromide

Solution

Ethidium bromide is a classic DNA-staining dye that intercalates between base pairs and fluoresces under ultraviolet light, allowing separated DNA fragments to be seen on an agarose gel. The other options are used for different purposes and do not specifically visualize DNA bands this way.

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• The separated DNA fragments can be visualised only after staining the DNA with Ethidium bromide followed by exposure to UV radiation.

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