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What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

1. The smaller the fragment size, the farther it moves
2. Positively charged fragments move to farther end
3. Negatively charged fragments do not move
4. The larger the fragment size, the farther it moves

Correct answer: The smaller the fragment size, the farther it moves

Solution

Agarose gel acts like a sieve, so smaller DNA fragments experience less resistance and travel farther through the gel. DNA is negatively charged and moves toward the positive electrode, but size determines how far it migrates.

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