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Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement. (a) The enzyme cuts DNA molecule at identified position within the DNA. (b) The enzyme binds DNA at specific sites and cuts only one of the two strands. (c) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand. (d) The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.

1. The enzyme cuts DNA molecule at identified position within the DNA.
2. The enzyme binds DNA at specific sites and cuts only one of the two strands.
3. The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
4. The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.

Correct answer: The enzyme binds DNA at specific sites and cuts only one of the two strands.

Solution

Restriction endonucleases recognize specific palindromic DNA sequences and cut the sugar-phosphate backbone at defined positions on both strands. The statement about cutting only one strand describes a nicking enzyme, not a typical restriction endonuclease.

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