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The two antibiotic resistance genes on vector pBR322 are for:

1. Tetracycline and Kanamycin
2. Ampicillin and Tetracycline
3. Ampicillin and Chloramphenicol
4. Chloramphenicol and Tetracycline

Correct answer: Ampicillin and Tetracycline

Solution

pBR322 is a standard cloning vector that contains two antibiotic resistance genes: bla for ampicillin resistance and tet for tetracycline resistance. These markers allow selection and identification of transformed cells and recombinants.

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