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Match the following enzymes with their functions:
Column-I
(A) Restriction endonuclease
(B) Restriction exonuclease DNA
(C) DNA ligase
(D) Tag polymerase
Column-II
(i) Joins the DNA fragments
(ii) Extends primers on genomic template
(iii) Cuts DNA at specific position
(iv) Removes nucleotides from the ends of DNA
Select the correct option from the following:
- (a) (A)-(ii), (B)-(iv), (C)-(i), (D)-(iii)
- (b) (A)-(iii), (B)-(i), (C)-(iv), (D)-(ii)
- (c) (A)-(iii), (B)-(iv), (C)-(i), (D)-(ii)
- (d) (A)-(iv), (B)-(iii), (C)-(i), (D)-(ii)
Correct answer: (c) (A)-(iii), (B)-(iv), (C)-(i), (D)-(ii)
Solution
Restriction endonucleases cut DNA at specific internal recognition sites, while restriction exonucleases remove nucleotides from DNA ends. DNA ligase seals DNA fragments together, and Taq polymerase extends primers during DNA synthesis.
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