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The specific palindromic sequence which is recognized by EcoRI is:

1. 5' - GGAACC - 3' 3' - CCTTGG - 5'
2. 5' - CCTTAGG - 3' 3' - GGAATCC - 5'
3. 5' - GGATCC - 3' 3' - CCTAGG - 5'
4. 5' - GAATTC - 3' 3' - CTTAAG - 5'

Correct answer: 5' - GAATTC - 3' 3' - CTTAAG - 5'

Solution

EcoRI recognizes a specific palindromic DNA sequence, meaning the 5'→3' sequence on one strand matches the 5'→3' sequence on the complementary strand when read in reverse. The site is 5'‑GAATTC‑3' / 3'‑CTTAAG‑5'.

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