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In order to obtain virus-free plants through tissue culture the best method is:

1. Embryo rescue
2. Anther culture
3. Meristem culture
4. Protoplast culture

Correct answer: Meristem culture

Solution

Meristem culture is best because the shoot apical meristem usually has little or no virus load, so regenerated plants are more likely to be virus-free. Other tissue culture methods do not specifically exploit this virus-free zone.

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