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Under which of the following conditions will there be no change in the reading frame of following mRNA?
5′ AACAGCGCGUGCUAUU 3′
- Insertion of G at 5th position
- Deletion of G from 5th position
- Insertion of A and G at 4th and 5th positions respectively
- Deletion of GGU from 7th, 8th and 9th positions
Correct answer: Deletion of GGU from 7th, 8th and 9th positions
Solution
A reading frame stays unchanged when the number of nucleotides inserted or deleted is a multiple of 3. Deleting GGU removes exactly three bases, so the codon grouping downstream is preserved.
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