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ExamsNEETBiology

E.coli cells with a mutated z gene of the lac operon cannot grow in medium containing only lactose as the source of energy because:

  1. the lac operon is constitutively active in these cells
  2. they cannot synthesize functional beta-galactosidase
  3. in the presence of lactose E.coli cells do not utilize lactose
  4. they cannot transport lactose from the medium into the cell

Correct answer: they cannot synthesize functional beta-galactosidase

Solution

The lacZ gene encodes beta-galactosidase, the enzyme that cleaves lactose into glucose and galactose. A mutated z gene means no functional beta-galactosidase, so the cells cannot use lactose as their sole energy source.

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