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E.coli cells with a mutated z gene of the lac operon cannot grow in medium containing only lactose as the source of energy because:
- the lac operon is constitutively active in these cells
- they cannot synthesize functional beta-galactosidase
- in the presence of lactose E.coli cells do not utilize lactose
- they cannot transport lactose from the medium into the cell
Correct answer: they cannot synthesize functional beta-galactosidase
Solution
The lacZ gene encodes beta-galactosidase, the enzyme that cleaves lactose into glucose and galactose. A mutated z gene means no functional beta-galactosidase, so the cells cannot use lactose as their sole energy source.
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