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During DNA replication, the strands separate by

1. DNA polymerase
2. topoisomerase
3. unwindase/Helicase
4. gyrase

Correct answer: unwindase/Helicase

Solution

Helicase (also called unwindase) is the enzyme that unwinds the DNA double helix and separates the two strands by breaking the hydrogen bonds between base pairs. DNA polymerase builds new DNA, while topoisomerase and gyrase relieve twisting stress ahead of the fork.

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