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During translation initiation in prokaryotes, a GTP molecule is needed in

1. association of 50 S subunit of ribosome with initiation complex
2. formation of formyl-met-tRNA
3. binding of 30 S subunit of ribosome with mRNA
4. association of 30 S mRNA with formyl-met-tRNA

Correct answer: association of 50 S subunit of ribosome with initiation complex

Solution

In prokaryotic translation initiation, IF-2 binds GTP and helps bring fMet-tRNA to the 30S initiation complex. GTP is then hydrolyzed when the 50S subunit joins, forming the complete 70S initiation complex.

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