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During DNA replication, Okazaki fragments are used to elongate:

1. The lagging strand towards replication fork.
2. The leading strand away from the replication fork.
3. The lagging strand away from the replication fork.
4. The leading strand towards replication fork.

Correct answer: The lagging strand away from the replication fork.

Solution

Okazaki fragments are synthesized on the lagging strand because DNA polymerase can only extend DNA in the 5' to 3' direction. Since the template runs opposite the fork’s movement, this strand is made in short pieces away from the replication fork.

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