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What will be the sequence of mRNA produced by the following stretch of DNA? 3'ATGCATGCATGCATG 5' TEMPLATE STRAND 5'TACGTACGTACGTAC 3' CODING STRAND

1. 3' AUGCAUGCAUGCAUG 3'
2. 3' AUGCAUGCAUGCAUG 5'
3. 5' UACGUACGUACGUAC 3'
4. 3' UACGUACGUACGUAC 5'

Correct answer: 3' AUGCAUGCAUGCAUG 5'

Solution

RNA polymerase reads the template strand 3'→5' and synthesizes mRNA 5'→3'. Using base-pair rules (A-U, T-A, G-C, C-G), the transcript matches the coding strand except U replaces T.

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