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The chemiosmotic coupling hypothesis of oxidative phosphorylation proposes that adenosine triphosphate (ATP) is formed because:

1. high energy bonds are formed in mitochondrial proteins
2. ADP is pumped out of the matrix into the intermembrane space
3. a proton gradient forms across the inner membrane
4. there is a change in the permeability of the inner mitochondrial membrane toward adenosine diphosphate (ADP)

Correct answer: a proton gradient forms across the inner membrane

Solution

Chemiosmotic coupling says electron transport pumps protons across the inner mitochondrial membrane, creating a proton-motive force. ATP synthase uses that gradient’s energy to convert ADP and phosphate into ATP.

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