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The number of substrate level phosphorylations in one turn of citric acid cycle is

1. One
2. Two
3. Three
4. Zero

Correct answer: One

Solution

The citric acid cycle has one substrate-level phosphorylation step: conversion of succinyl-CoA to succinate, which generates GTP (or ATP in some tissues). The other energy-yielding steps produce NADH or FADH2, not direct phosphorylation.

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