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The introduction of t-DNA into plants involves:

1. Altering the pH of the soil, then heat shocking the plants
2. Exposing the plants to cold for a brief period
3. Allowing the plant roots to stand in water
4. Infection of the plant by Agrobacterium tumefaciens

Correct answer: Infection of the plant by Agrobacterium tumefaciens

Solution

t-DNA is the segment of the Ti plasmid from Agrobacterium tumefaciens that naturally integrates into plant genomes during infection. This bacterium is widely used as a vector for plant genetic transformation.

Related NEET Biology questions

• The sequence that controls the copy number of the linked DNA in the vector, is termed:
• For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:
• Biolistics (gene-gun) is suitable for:
• PCR and Restriction Fragment Length Polymorphism are the methods for:
• Which one is a true statement regarding DNA polymerase used in PCR
• The separated DNA fragments can be visualised only after staining the DNA with Ethidium bromide followed by exposure to UV radiation.

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