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Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

1. Lipase
2. Protease
3. Endonuclease
4. Exonuclease

Correct answer: Endonuclease

Solution

Endonucleases cleave phosphodiester bonds at internal sites within a polynucleotide chain. Exonucleases remove nucleotides from the ends, while lipases and proteases act on lipids and proteins, respectively.

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