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For gene transfer into the host cell without using vector microparticles made of tungsten and gold coated with foreign DNA are bombarded into target cells at a very high velocity. This method is called:

1. a) Microinjection
2. b) Electroporation
3. c) Biolistics
4. d) Liposome-mediated gene transfer

Correct answer: c) Biolistics

Solution

Biolistics is the particle bombardment method in which DNA-coated gold or tungsten microprojectiles are shot into cells. It is specifically used for gene transfer without a biological vector.

Related NEET Biology questions

• The sequence that controls the copy number of the linked DNA in the vector, is termed:
• For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:
• Biolistics (gene-gun) is suitable for:
• PCR and Restriction Fragment Length Polymorphism are the methods for:
• Which one is a true statement regarding DNA polymerase used in PCR
• The separated DNA fragments can be visualised only after staining the DNA with Ethidium bromide followed by exposure to UV radiation.

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