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The correct option is (a) because ori sequence is responsible for controlling the copy number of the linked DNA in the vector. Ori i.e. origin of replication is responsible for initiation of replication.

1. The correct option is (a) because ori sequence is responsible for controlling the copy number of the linked DNA in the vector. Ori i.e. origin of replication is responsible for initiation of replication.
2. The high AT-content results in the low thermodynamic stability of the region which accounts for its role in the process of replication initiation. At the AT-rich regions, the initial DNA helix destabilization (opening) is induced by binding an initiator protein to its respective recognition sequences situated nearby.
3. Selectable marker is a gene introduced into a cell, especially a bacterium or to cells in culture that confers a trait suitable for artificial selection. Selectable markers help in identification and elimination of non-transformants whilst permitting selective growth of transformants.
4. Use of nptII in concert with the antibiotic kanamycin has become the most widely used selectable marker.

Correct answer: The correct option is (a) because ori sequence is responsible for controlling the copy number of the linked DNA in the vector. Ori i.e. origin of replication is responsible for initiation of replication.

Solution

The ori is the origin of replication, so it is the DNA sequence that allows replication to begin in the host cell. Because replication starts there and its properties affect how often the plasmid is copied, option (a) is the best match.

Related NEET Biology questions

• The sequence that controls the copy number of the linked DNA in the vector, is termed:
• For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:
• Biolistics (gene-gun) is suitable for:
• PCR and Restriction Fragment Length Polymorphism are the methods for:
• Which one is a true statement regarding DNA polymerase used in PCR
• The separated DNA fragments can be visualised only after staining the DNA with Ethidium bromide followed by exposure to UV radiation.

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