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Which one of the following represents a palindromic sequence in DNA?

1. 5' - GAATTC - 3' 3' - CTTAAG - 5'
2. 5' - CTTAAG - 3' 3' - GAATTC - 5'
3. 5' - CATTAG - 3' 3' - GTAATC - 5'
4. 5' - GATAAC - 3' 3' - CATTAG - 5'

Correct answer: 5' - GAATTC - 3' 3' - CTTAAG - 5'

Solution

A DNA palindrome is a sequence that is identical to its reverse complement. For 5' - GAATTC - 3', the complementary strand is 3' - CTTAAG - 5', and reading that strand 5'→3' gives GAATTC again.

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