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Which step of translation does not consume a high energy phosphate bond?

1. Translocation
2. Amino acid activation
3. Peptidyl-transferase reaction
4. Aminoacyl tRNA binding to active ribosomal site

Correct answer: Peptidyl-transferase reaction

Solution

The peptidyl-transferase reaction forms the peptide bond between amino acids on the ribosome, and it does not directly use a high-energy phosphate bond. The energy for this bond comes from the aminoacyl-tRNA linkage created during amino acid activation, which does consume ATP.

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