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Which one of the following is not applicable to RNA?

1. 5’ phosphoryl and 3’ hydroxyl ends
2. Heterocyclic nitrogenous bases
3. Chargaff’s rule
4. Complementary base pairing

Correct answer: Chargaff’s rule

Solution

Chargaff’s rule describes the base composition relationships in double-stranded DNA, where adenine equals thymine and guanine equals cytosine. RNA does have phosphate-sugar ends, nitrogenous bases, and can form complementary base pairs, but it does not generally obey Chargaff’s rule.

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