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Poisons like cyanide inhibit Na⁺ efflux and K⁺ influx during cellular transport. This inhibitory effect is reversed by an injection of ATP. This demonstrates that

1. ATP is the carrier protein in the transport system.
2. energy for Na⁺ – K⁺ exchange pump comes from ATP.
3. ATP is hydrolysed by ATP to release energy.
4. Na⁺ – K⁺ exchange pump operates in the cell.

Correct answer: energy for Na⁺ – K⁺ exchange pump comes from ATP.

Solution

Cyanide blocks ATP production, so active transport of Na⁺ and K⁺ stops. When ATP is injected and the transport resumes, it shows the pump depends on ATP-derived energy to work.

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