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Assuming human pupil to have a radius of 0.25 cm and a comfortable viewing distance of 25 cm, the minimum separation between two objects that human eye can resolve at 500 nm wavelength is:

1. 1 μm
2. 30 μm
3. 100 μm
4. 300 μm

Correct answer: 30 μm

Solution

The minimum separation that the human eye can resolve is determined by the diffraction limit, which is influenced by the pupil size and the wavelength of light. Using the formula for angular resolution, the given parameters yield a resolution limit of approximately 30 μm, making it the correct answer.

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