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A reagent suitable for the determination of N-terminal residue of a peptide is

1. p-Toluenesulphonyl chloride
2. 2, 4-Dinitrophenylhydrazine
3. Carboxypeptidase
4. 2, 4-Dinitrofluorobenzene

Correct answer: 2, 4-Dinitrofluorobenzene

Solution

2,4-Dinitrofluorobenzene (DNFB) is used in Sanger's method to identify the N-terminal amino acid of a peptide by forming a stable dinitrophenyl derivative.

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