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Engelmann conducted an experiment using a prism to separate light into its spectral colors and illuminated a green alga, Cladophora, surrounded by aerobic bacteria. These bacteria helped identify where oxygen was being produced. What conclusion did Engelmann draw from this experiment?

1. Azotobacter is an aerobic bacterium, and Cladophora is a type of green alga.
2. Engelmann used a prism to divide light into its spectral colors.
3. The bacteria were employed to locate the regions of oxygen production.
4. The first action spectrum for photosynthesis was determined.

Correct answer: The first action spectrum for photosynthesis was determined.

Solution

The first action spectrum of photosynthesis was observed by Engelmann when he used aerobic bacteria to detect oxygen evolution in blue and red light regions, confirming the role of these wavelengths in photosynthesis.

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