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In Lederberg’s replica experiment what shall be used to obtain streptomycin-resistant strain?

1. minimal medium and streptomycin
2. complete medium and streptomycin
3. only minimal medium
4. only complete medium

Correct answer: minimal medium and streptomycin

Solution

In Lederberg’s replica plating, colonies are first grown on a nonselective plate, then copied onto a plate containing streptomycin. Only cells with pre-existing resistance survive there, allowing the resistant strain to be isolated.

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