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Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as adaptation. These imprints need to be used

1. on plates with and without streptomycin
2. on plates with minimal medium
3. only on plates with streptomycin
4. only on plates without streptomycin

Correct answer: on plates with and without streptomycin

Solution

Replica plating works by transferring the same colony pattern onto both streptomycin-containing and streptomycin-free plates. Resistant colonies appear at the same positions on the selective plate as on the control, showing the mutation existed before exposure rather than being induced by adaptation.

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