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Which one of the following experiments suggests that simplest living organisms could not have originated spontaneously from non-living matter?

1. Larvae could appear in decaying organic matter.
2. Microbes did not appear in stored meat.
3. Microbes appeared from unsterilized organic matter.
4. Meat was not spoiled, when heated and kept sealed in a vessel.

Correct answer: Meat was not spoiled, when heated and kept sealed in a vessel.

Solution

If heated meat kept sealed does not spoil, it shows microorganisms were not generated spontaneously in the meat. Instead, spoilage requires contamination from preexisting living organisms, which argues against spontaneous generation.

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