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Which antibiotic inhibits interaction between tRNA and mRNA during bacterial protein synthesis?

1. Neomycin
2. Streptomycin
3. Tetracycline
4. Erythromycin

Correct answer: Tetracycline

Solution

Tetracycline binds the bacterial 30S ribosomal subunit and blocks attachment of aminoacyl-tRNA to the A site, stopping codon-anticodon interaction during translation. That directly prevents tRNA from properly engaging with mRNA on the ribosome.

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