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Auxin can be bioassayed by:

1. Hydroponics
2. Potometer
3. Lettuce hypocotyl elongation
4. Avena coleoptile curvature

Correct answer: Avena coleoptile curvature

Solution

Auxin was historically quantified using the Avena coleoptile curvature test, where unequal auxin distribution causes bending. This makes the response easy to measure and directly linked to auxin activity.

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